Paclobutrazol residues in soil can change soil physicochemical properties and inhibit thegrowth of soil microorganisms and plants. Enzyme remediation has received widespread attentionin the removal of persistent organic pollutants due to its advantages such as environmentaladaptability and high stability. In this study, different enzymes were combined to investigatetheir degradation of PBZ in soil and further determine the optimal combination of enzymes and thebest reaction conditions. The results showed that laccase, amylase, peroxidase and lipase amongthe selected enzymes showed degradation ability against PBZ, with the degradation rates of35.47%,14.25%,24.51% and 11.80%, respectively. The best enzyme combination for PBZdegradation was selected as laccase + peroxidase by combining different enzymes, achieving acombined degradation efficiency of 65.06% and demonstrating synergistic degradation effects.The environmental factors affecting the degradation efficiency of enzymes were further optimized.and the highest degradation rate reached 88.42% under the conditions of 45% soil moisturecontent, soil pH of 5.5, temperature of 40 ℃ and air ventilation.